FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_347.351000000a0f06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_347.351000000a0f06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116974
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCCATTTTTCATCTGCAACACAGAAAGTTGGGGGGAGTT2790.2385145416930258No Hit
GTAGTAACAAGAGCGGCTAAAAATCTACTTATTTAACAAAAATCCCAAAT1780.1521705678184896No Hit
AAGCAGAGCAGACCACAAACAGAATAAACAAAAGGTAAATTGTTGGACTT1750.14960589532716673No Hit
GTAGTAACAAGAGCTATGTTTAGGTCTCTCTCTCCTTGACACCTGACACA1560.13336296954878862No Hit
ACAACAGCTAACACCACAGACTTCTCAACTGGTAGCTTATACAATTCTGA1450.12395917041393813No Hit
TGTCCTCCAGATTCACCTACATTGTGGCAAATAGCAGGAGCCCTCTTTTT1410.12053960709217434No Hit
GTATATAAAGAATCTCTTAATTATTGCTGCTTTCAATATTCTCTCTGTAT1380.11797493460085147No Hit
CACGAGCATAGAGGACCATTTCCAATCTCTCATTCCTTATCTTTTCATAG1310.11199069878776481No Hit
GAGTAACACCTGGGAAAGCTTCACCAGAGGGTCCTTTTCCACCACAGCAA1300.11113580795732383No Hit
GCGGTGCAGACCACAAACAGAATAAACAAAAGGTAAATTGTTGGACTTAA1250.10686135380511907No Hit
CTTTATATACAATGGAGGCTTAATAGTGTTAACAGCTGACTTCTCATCAG1240.10600646297467813No Hit
CACTAGACAAATACTTTACTTTTATTTTTTTTATTTCAGTAGGTTTTTGG1200.10258689965291432No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA2650.0128.58491
TAGTAAC2800.0119.107142
GTAACAA2900.0117.500014
GCACGCA950.0106.842112
CGCACTT1100.0105.4545445
CGAGCAT352.7464012E-7103.571433
AGCACGC1000.0101.51
TAACAAG3500.097.357155
AAGAGCG451.0832082E-896.6666649
AAGCGGA259.365885E-487.01
AGTAACA4500.074.1111153
AACACGA1700.072.51
ACGAGCA851.6734703E-1068.23532
ACGCACT1600.067.968754
CGAGCTA651.3872886E-766.923085
GAGCATA554.039239E-665.909094
ACGAGCT1100.065.909094
GAACCTC451.1894718E-464.444446
CACGCAC1600.063.43753
AACAAGA5400.063.101856
Produced by FastQC (version 0.11.2)