FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_346.351000000a0ef8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_346.351000000a0ef8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149897
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACTGTACCCAATGTGTAGTCTAGACAAAGATTTTAAATCAACTA1550.10340433764518303No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTT553.6379788E-12105.4545445
ACGCACT651.4551915E-1189.230774
AACACGA651.4551915E-1189.230776
GCACGCA702.910383E-1182.857142
CACGAGC755.4569682E-1177.333338
ACGAGCC300.00193252172.59
AAACACG955.456968E-1268.6842045
ACACGAG902.8012437E-1064.444447
ACGAGCT350.003560591362.1428579
CACGCAC954.5110937E-1061.0526283
AGCACGC1007.1304385E-1058.01
GTAGTAA1205.820766E-1154.3751
ACGAGCA450.00962327548.3333325
GCACTTG951.909897E-645.789476
CCGTCTG657.3165563E-444.6153839
GAAACAC2050.042.4390264
TAACAAG1803.1614036E-936.255
GTAACAA1604.5452907E-836.254
GTAGAAA3450.035.724641
GGGTATA850.002734587834.117651
Produced by FastQC (version 0.11.2)