FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_344.351000000a0eeb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_344.351000000a0eeb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37500
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGACCCAAAAGGAGCAATAGAAAGAAATCTAGCAAGGATGTCTCCC740.19733333333333333No Hit
AGACAATATAGACCCAAAAGGAGCAATAGAAAGAAATCTAGCAAGGATGT620.16533333333333333No Hit
ACATAGACAATATAGACCCAAAAGGAGCAATAGAAAGAAATCTAGCAAGG600.16No Hit
TAGTAGTACAGACCCAAGAGTGGACTTGGGAAAGTGGCCAAAATATACTG540.14400000000000002No Hit
ATATTGTCTATGTAGCTCCTCATTCCTTCGAAATTGGAGAAACCAGCTGG510.136No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC510.136No Hit
ATACAGAAGATTGGAATATGGGACGGAGAAGAGGAGTTCCATGTGAGATG500.13333333333333333No Hit
GTATGTAGAAACCAGCCTGCTGCAACGGCACTAAACACAACAATAACCTC460.12266666666666667No Hit
GTAGTTGATTTCATTAACAAAGTATTTCCTTCTTTCACTCCCTACATGCT420.11199999999999999No Hit
GTAATAAGTGACATGAATTTTCTTGATGAAGAAGGAAAAGCATATACAGC410.10933333333333334No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCA100.0070638647145.04
TAACCAG100.0070638647145.05
GTATATT100.0070638647145.03
GCACTTC203.8367882E-4108.756
CCGTTAC406.579977E-572.51
CGTTACA501.9852846E-458.02
GTGTAAA501.9852846E-458.01
GTATGTG651.07337055E-555.7692349
GTAGTAA651.07337055E-555.7692341
TAAATAC701.6646192E-551.7857134
ACACAGC604.884037E-448.3333326
GTTACAC604.884037E-448.3333323
TAACAAG752.5035255E-548.3333325
GAAGTAT604.884037E-448.3333326
GAGCAGA450.00956749848.3333321
GTAACAA855.24341E-542.6470574
AAGTATG700.001043672441.428577
AGTAACA1053.7263271E-641.428573
CACAGCA907.34537E-540.277787
TAGTAAC907.34537E-540.277782
Produced by FastQC (version 0.11.2)