FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_340.351000000a0ede.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_340.351000000a0ede.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105896
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACGA259.363411E-487.06
AAGAGCG451.1889517E-464.444449
TAACCTA350.003557701862.1428575
GCGGAGC350.003557701862.1428574
AAACACG502.0020065E-458.05
CGTGACT400.006036008754.3753
ACACGAG450.00961552648.3333327
TAGACAG450.00961552648.3333325
AAGCGGA450.00961552648.3333322
TAGATAG450.00961552648.3333325
TAGTAAC901.3122917E-648.3333322
GTATAGA855.309066E-542.6470571
CTAATAT700.00105236141.428573
GTAGTAA1157.0824317E-637.8260881
CAGAAGC1655.930451E-835.1515162
TAGAAAC1655.930451E-835.1515162
CATAGAC850.00273097634.1176452
TCTAATA1352.124272E-532.222222
TATAGAA1601.8562314E-631.718752
GTAACAA1402.723734E-531.0714284