FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_338.351000000a0ec1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_338.351000000a0ec1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109594
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA1340.12226946730660437No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG1320.12044454988411775No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA1190.10858258663795463No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATTATCGTTTTGT1180.10767012792671131No Hit
CACAAGCACTGCCTGCTGTACACTTCAACCACATCCTTCATTTCATACAA1120.10219537565925142No Hit
GTATGTAGAAACCAGCCTGCTGCAACAGCACTAAACACAACAATAACCTC1110.1012829169480081No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATCTC100.007082308145.05
ACACGAG352.7452188E-7103.571437
AACACGA406.0809543E-790.6256
GCGGAGC259.3642913E-487.01
GCGGTGC300.00193112872.51
GAAACAC554.0375326E-665.909094
AAACACG554.0375326E-665.909095
CACGAGC606.7653546E-660.4166688
ACGAGCA400.006036570854.3759
CAGAAGC553.206345E-452.7272723
TACCCTC450.00961641648.3333326
CCCTCGT450.00961641648.3333328
GATGTCG450.00961641648.3333326
CCTCGTT450.00961641648.3333329
ACCCTCG450.00961641648.3333327
GTATGTA1203.5706762E-948.3333321
GAGCAGA450.00961641648.3333321
ATACCCT450.00961641648.3333325
TAGAAAC1401.3948011E-841.428576
CCCATAC700.001052522441.428571