FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_337.351000000a0ea8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_337.351000000a0ea8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences178037
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGCG151.2283205E-4145.05
ACGCGCC100.0070860023144.999986
GTAGTAA3400.0104.48531
TAGTAAC3350.0103.880592
CGCACTT1400.0103.571435
GCACGCA1450.095.02
GTAACAA3800.091.578954
AGCACGC1550.088.870971
ACGCACT1750.087.04
CACGCAC1800.080.555563
TAACAAG4500.077.3333365
AGTAACA4900.071.020413
AAGAGCG902.8012437E-1064.444449
CGAGCTT451.19117416E-464.444445
AACACGA2500.058.01
CAAGAGC6250.056.848
ACAAGAG6250.056.847
AAGCGGA701.69031E-551.7857131
GCACTTG1850.050.945956
GTAGAAA3900.050.192311