FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_333.351000000a0e9b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_333.351000000a0e9b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149465
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAGAAACCAGCCTGCTGCAACGGCACTAAACACAACAATAACCTC4730.31646204797109695No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA4080.2729736058609039No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT2740.18332050981835213No Hit
TCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACACT2630.17596092730739638No Hit
CCCATACACTGGTGTTCCCCCTTACTCCCATGGAACGGGAACAGGCCACA2460.16458702706319206No Hit
GTATTTAGTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGA2400.16057270932994347No Hit
GTTAAAACAGGCCTCAAAAAGGTTGCAACACTTAGCGTAGCATTGTTCCT2350.1572274445522363No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC1950.13046532633057906No Hit
GTTAGCTATTATCGTTTTGTGGAACAATATGTTATCATCAATCCTTTTAC1930.1291272204194962No Hit
TAGTAGTACAGACCCAAGAGTGGACTTGGGAAAGTGGCCAAAATATACTG1860.12444384973070619No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGAATTGAAAAGAACCCTT1850.12377479677516474No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT1820.12176763790854045No Hit
GTACAATGGTAGACCCAACAAATGGACCATTACCTGAAGACAATGAGCCA1810.12109858495299904No Hit
TCTCAGGCTTGTCCAATGAATCAATGATATCTTGGCAAAAGGGTACCAAT1790.11976047904191617No Hit
ATTTAGTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGATA1780.11909142608637474No Hit
ATCCAATGATGACCAATAACCCCATATACATCTTCGGAGCTTATGTGTAC1730.11574616130866759No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA1690.11306994948650186No Hit
GGTTTTAGCTTTTCAAGTTCTTCAATGGTTTTGAAATCTCCAAGATCTGG1660.11106279061987756No Hit
CCCCATATACATCTTCGGAGCTTATGTGTACCCAATCTCACCAAGGTGAG1640.10972468470879469No Hit
CTGTTAAAACAGGCCTCAAAAAGGTTGCAACACTTAGCGTAGCATTGTTC1630.10905563175325328No Hit
GTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGATACTGCA1630.10905563175325328No Hit
TTCTTAATCTATGGGCCATAGCTTCAAGCATGCTGTGCTGACCTACTGGT1610.10771752584217041No Hit
GTATTCAGGGTCCATTAGATTGTACTTCAACACTATTTCTGGGATATGCA1590.10637941993108754No Hit
CCCATACAGGCAGCAATTTCAACAACATTCCCATACACTGGTGTTCCCCC1550.10370320810892182No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCACT100.007084872145.04
GCACGCA100.007084872145.02
TAACAGC100.007084872145.06
CCGAAGC100.007084872145.03
ATACGGA100.007084872145.0145
CGGAGCG100.007084872145.02
AGCACGC100.007084872145.01
GAAACAC650.0111.538474
AAACACG750.096.666675
GCGGAGC406.644675E-572.51
AACACGA903.6379788E-1272.56
ACACGAG1153.8198777E-1156.7391327
GAAGCGG400.00604086154.3754
AGAAGCG400.00604086154.3753
TTTTCGA400.00604086154.375145
CACGAGC1258.913048E-1152.2000058
ACGAGCC803.7193306E-545.31259
GTCATAA657.3164934E-444.6153871
AGCTCAG657.3164934E-444.6153877
TATAGAG855.319631E-542.6470575
Produced by FastQC (version 0.11.2)