FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_328.351000000a0e8e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_328.351000000a0e8e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155290
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTAGTACAGACCCAAGAGTGGACTTGGGAAAGTGGCCAAAATATACTG3800.24470345804623608No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC2860.18417155000321977No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT2170.13973855367377164No Hit
GTGTCCTTTTATTCATCCATTATTTCATCTACTGATTCTAATACCTTACT2120.13651877133105803No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA2050.13201107605125892No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT1950.12557151136583167No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATTATCGTTTTGT1880.12106381608603259No Hit
GCTTTGGTCATGTCATATCCTTGTATCGATGATTCTTGTTCAACAATTGC1690.10882864318372079No Hit
GGTTTTAGCTTTTCAAGTTCTTCAATGGTTTTGAAATCTCCAAGATCTGG1640.10560886084100714No Hit
CTTTACATACAGTCCCATCCCCATTCTTTTGGTTTTAGACATCGTTCGAG1620.1043209479039217No Hit
GTACTACTAAATTCGAAAGTTACAACTGTTACAACATCAGTATCTCCTCT1610.10367699143537897No Hit
ATGTTAATCCATCCCCTGTGGCCCACTTGGCATAATTGGTTTTCTGTAAT1600.10303303496683625No Hit
GTTTAGGATTGGCTCCCTATTTGTGAGTGGGAGGGAAAAATCTGTGTACC1570.10110116556120807No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACACG650.0122.692315
AACACGA650.0111.538466
ACACGAG750.096.6666647
CACGAGC750.096.6666648
GGCTAAC301.595186E-596.6666641
ACGAGCA950.076.315799
AGCGGAG406.645486E-572.500016
GAAACAC1100.072.54
CGGAGCC300.001932653172.58
CCCGTAA350.00356083262.142861
CCGTAAC350.00356083262.142862
GCTAACA350.00356083262.142862
AGAAGCG902.0345396E-856.3888935
TAACCTT400.006041299654.3750044
CGTAACC400.006041299654.3750043
AAGCGGT553.2105256E-452.7272727
GCGGAGC553.2105256E-452.7272727
CAGTAGG450.009623921548.3333361
GCGGTGC604.932098E-448.3333329
GAGCAGA1402.6921043E-1046.6071471
Produced by FastQC (version 0.11.2)