FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_327.351000000a0e71.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_327.351000000a0e71.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170153
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT3400.1998201618543311No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT2400.14104952601482196No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT2340.1375232878644514No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT2200.12929539884692012No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG2150.12635686705494467No Hit
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT2130.12518145433815447No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA2050.12047980347099375No Hit
GTTTAGTATACCATTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA2020.11871668439580847No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC1940.11401503352864775No Hit
TCTGCATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA1850.10872567630309192No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT1850.10872567630309192No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC1820.10696255722790664No Hit
ATACTAAACAGATCAGGACAAGGTATTTGAGCCTTTAGTCTTTTTGTTTT1820.10696255722790664No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC1770.10402402543593119No Hit
CACAAGCACTGCCTGCTGTACACTTCAACCACATCCTTCATTTCATACAA1770.10402402543593119No Hit
TAGTAGTACAGATCCAAGAGTGGACTCAGGAAAGTGGCCAAAATATACTG1710.10049778728556064No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCAAC100.007085728145.0145
GGGGCCT100.007085728145.04
GAGCAGA403.274181E-11126.8751
TGTCCAG203.8604846E-4108.751
CCAGGCT203.8604846E-4108.754
GAAACAC750.096.666674
ACGAGCC301.5956282E-596.6666649
CACAGGA259.37327E-487.03
AACACGA750.087.06
TTATGTG259.37327E-487.05
AAACACG850.085.294125
GTAACAA1500.082.166674
ACACGAG851.8189894E-1276.764717
TCCAGGC300.001932972872.53
CAGGATT300.001932972872.55
TAGAAAC1150.069.347832
CACGAGC955.456968E-1268.684218
AGAAGCG554.0471477E-665.9090965
GTGATTT350.003561420162.1428579
GAAGCGG606.781427E-660.4166686
Produced by FastQC (version 0.11.2)