FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_325.351000000a0e64.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_325.351000000a0e64.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125903
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTAAC955.456968E-1268.684212
CACGCAC702.3180291E-762.1428573
ACGCACT851.2931196E-859.705884
CGCACTT753.7346763E-758.05
GTCCTAT400.006038656554.3751
GGGGCGT400.006038656554.3759
AGCACGC805.832353E-754.3751
GCACGCA858.8621164E-751.1764682
AGTAACA1301.2914825E-1050.192313
TCTGCGT604.928518E-448.333332145
AAGCACG450.00961972948.3333325
CTTAGTA855.3148317E-542.6470579
GTAGTAA1703.8198777E-1142.6470571
TAACAAG1451.9050276E-840.0000045
GTAACAA1451.9050276E-840.0000044
TGTACCA750.001478461138.6666645
ACTTAGT951.0237795E-438.1578948
CCCTATC800.002029872536.251
GAGTCTA900.003616290432.222229
CAGAAGC2051.1219527E-831.8292682
Produced by FastQC (version 0.11.2)