FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_323.351000000a0e58.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_323.351000000a0e58.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137855
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGTA100.00708428145.08
CGGTGCG100.00708428145.05
ATAGTCG100.00708428145.07
CGCACTT403.274181E-11126.8755
GTAGTAA1650.074.696971
ACGCACT808.018105E-963.43754
TAGTAAC2000.061.6252
AAACACG1301.8189894E-1255.769235
ACGAGCC400.006039869554.3753
GCACGCA953.1128366E-853.4210552
GTAACAA2400.051.3541684
AAGAGCG450.00962165148.3333329
GCGGTGC604.93016E-448.3333324
AAGCGGT604.93016E-448.3333322
AGCACGC1056.848495E-848.3333321
ATTAGTA657.3145103E-444.6153831
TAACAAG2800.044.0178575
AGTAACA2850.043.2456133
AACACGA1351.0149961E-842.9629636
ACGAGCT700.001053470941.428572
Produced by FastQC (version 0.11.2)