FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_322.351000000a0e4b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_322.351000000a0e4b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151235
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATAAAGAATCTCTTAATTATTGCTGCTTTCAATATTCTCTCTGTAT1570.10381194829239264No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTT950.0122.105275
GTAGTAA3450.0105.0724641
TAGTAAC3450.0105.0724642
AGCACGC1100.098.863631
GTAACAA3700.097.972984
ACGCACT1250.092.7999954
GCACGCA1250.087.02
CGAGCTT353.4278295E-582.857146
TAACAAG4800.075.5208365
CACGCAC1500.072.53
ACGAGCG300.00193255572.54
AGTAACA5000.072.53
GTAGCTA451.1905958E-464.444448
CGTAAGC350.003560653262.142857145
ACAAGAG6200.058.4677437
CAAGAGC6100.057.0491838
ACGAGCT651.0891861E-555.7692345
TCTCGCT553.210257E-452.7272724
AACAAGA6900.052.5362326
AAGAGCG752.5402358E-548.3333329
Produced by FastQC (version 0.11.2)