FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_320.351000000a0e3e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_320.351000000a0e3e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100030
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT1030.10296910926721983No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACTT650.0111.538475
GTAACAA1950.096.666674
TAGTAAC1950.096.666672
GCGGTGC700.093.214291
ACGAGCT406.0770435E-790.6255
AGCACGC800.090.6251
TAACAAG2150.087.6744165
CGGTGCA502.2477252E-886.999992
GTAGTAA2200.085.681821
ACGCACT850.085.294124
GCACGCA801.8189894E-1281.56252
AGTAACA2400.078.5416643
CACGCAC1009.094947E-1265.249993
ACAAGAG3150.059.841277
TTAATGT400.006035036454.3754
GTATGTA400.006035036454.3751
TGCACGA400.006035036454.3755
TTTACCG450.00961398248.333332145
GTCTCGC450.00961398248.3333321
AAACACG1056.823029E-848.3333325
Produced by FastQC (version 0.11.2)