FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_318.351000000a0e21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_318.351000000a0e21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165777
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA2500.075.41
AAGAGCG1100.072.59
AACACGA754.822141E-967.6666646
TAGTAAC2850.066.140352
AAACACG902.8012437E-1064.444455
ACGAGCA451.1909328E-464.444459
GTAACAA2850.063.5964934
ACGCACT1051.6370905E-1162.1428574
AAGCGGT350.00356125862.1428577
CGCACTT954.5292836E-1061.0526355
CATATAG502.0053332E-458.03
GCACGCA1051.0986696E-955.2380942
AAGCACG400.006042018554.3755
AGTAACA3600.052.3611153
AGAAGCG701.6898008E-551.7857135
CACGAGC1004.6713467E-850.758
AGCACGC1152.4629117E-950.4347841
GCGGTGC450.00962506248.3333369
ACACGAG1056.859773E-848.3333327
CACGCAC1402.6921043E-1046.6071433
Produced by FastQC (version 0.11.2)