FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_316.351000000a0e14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_316.351000000a0e14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191582
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCGAATATATTGCTAAAATTCTCACCAACAGAAATAACTGCACCAACAA4440.231754548965978No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT2960.15450303264398535No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC2750.14354166884154043No Hit
GGCGATATGGCAAACAGAATCCCCTTGGAATACAAAGGGATACAACTTAA2630.13727803238300051No Hit
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT2230.11639924418786735No Hit
TAGTAGTACAGATCCAAGAGTGGACTCAGGAAAGTGGCCAAAATATACTG2190.11431136536835403No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT2160.11274545625371904No Hit
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT2160.11274545625371904No Hit
GTTTAGTATACCATTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA2080.1085696986146924No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC2060.10752575920493575No Hit
ATATTGTCTATGTAGCTCCTCATTCCTTCAAAATTGGAGAAACCGGCTGG2040.1064818197951791No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT2040.1064818197951791No Hit
ATTCGAATATATTGCTAAAATTCTCACCAACAGAAATAACTGCACCAACA1980.10335000156590911No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT1950.10178409245127412No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT1940.1012621227463958No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTG100.007086419145.07
CAGCATC151.2284654E-4145.04
GTAAGTG100.007086419145.02
CGGAGCG100.007086419145.02
CGGTGCA500.0130.52
GGTGCAT500.0130.53
GTAACAA2400.0120.8333364
GAAACAC1450.0115.04
GCGGAGC203.8612346E-4108.751
AAACACG1600.0104.218755
TAGAAAC1850.097.972982
CAGCCTT301.596144E-596.6666646
TAACAAG3300.087.8787845
ACAAGAG3450.084.0579767
GAGCAGA353.4301727E-582.857141
ACGAGCC702.910383E-1182.857149
ACACGAG1850.082.29737
AACACGA1850.082.29736
TAGTAAC4150.075.120482
CACGAGC2100.072.58
Produced by FastQC (version 0.11.2)