FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_310.351000000a0e07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_310.351000000a0e07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences175908
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACTGTACCCAATGTGTAGTCTAGACAAAGATTTTAAATCAACTA1810.10289469495418059No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACGA100.0070859306145.05
CGCACTT750.096.6666645
ACGCACT1000.072.54
AACACGA1102.5465852E-1159.3181846
GTAGTAA1650.057.1212121
AAGCGGA553.2117017E-452.7272761
AGCACGC1457.2759576E-1250.01
GCACGCA1509.094947E-1248.3333322
AAGCGGT450.009626036548.3333327
ACACGAG1402.6921043E-1046.6071437
AAACACG1402.6921043E-1046.6071435
GAAGCGG657.3200435E-444.6153836
ACGAGCA657.3200435E-444.6153839
CACGAGC1557.3305273E-1042.0967758
GCGGTGC700.001054265941.428579
TAGTAAC2300.040.9782642
GTAGAAA2750.039.5454561
AGATAGT800.002032063736.256
GTAACAA2650.035.5660364
CACGCAC2103.7289283E-1034.5238083
Produced by FastQC (version 0.11.2)