FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_306.351000000a0df9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_306.351000000a0df9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences190404
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCTTTTTTTCATTTTAATCATTTGTTTGTCACATGTA3570.1874960610071217No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA3490.18329446860360077No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT3150.1654377008886368No Hit
GTATGTAGAAACCAGCCTGCTGCAACGGCACTAAACACAACAATAACCTC2950.15493371987983445No Hit
GCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAATG2950.15493371987983445No Hit
CTATAGAACATGCTGGTGAATTCAAACATTCCAGTTTCGTTGCAGTAACT2890.15178252557719374No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAAAACAATT2790.1465305350727926No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGCTCAGTAAAAACAATT2710.14232894266927165No Hit
TAGTAGTACAGACCCAAGAGTGGACTTGGGAAAGTGGCCAAAATATACTG2570.13497615596311002No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC2430.1276233692569484No Hit
AGCATATACTGACACGTACCATTCCTATGCAAAAAACATCCTAAGGACAC2360.12394697590386757No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGAATTGAAAAGAACCCTT2310.121320980651667No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG2230.11711938824814606No Hit
GTAGAAACACGAGCCTTTTTTTCATTTTAATCATTTGTTTGTCACATGTA2200.11554379109682571No Hit
TCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACACC2140.11239259679418499No Hit
ATATTGTCTATGTAGCTCCTCATTCCTTCGAAATTGGAGAAACCAGCTGG1980.10398941198714313No Hit
GCGGTGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAATG1950.10241381483582278No Hit
AAGCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAA1930.10136341673494254No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCCTT100.007086387145.05
ACAGCGT100.007086387145.04
CACAGCG100.007086387145.03
CGGTGCA351.0913936E-11145.02
GGTGCAT306.475602E-10145.03
GAAACAC1350.0118.148154
AAACACG1500.0106.333335
GCGGAGC700.093.214291
AACACGA1700.089.558826
GCGGTGC1400.077.678571
GTAACAA3550.077.605644
ACACGAG2000.076.1257
GTAGTAA4050.069.814811
CACGAGC2200.069.204558
TAACAAG4050.068.024695
AGCGTTT554.0489977E-665.9090966
TAGTAAC4400.065.9090962
AGAAGCG451.1913869E-464.444443
TGGGGGG350.003562071662.1428575
ACGAGCA1800.060.4166649
Produced by FastQC (version 0.11.2)