FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_302.351000000a0dec.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_302.351000000a0dec.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118471
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCGGA100.0070830286145.000021
CGCACTT406.0840284E-790.625015
CGTGGTG259.366184E-487.0145
CGAGCAT259.366184E-487.03
ACGCACT451.2261626E-680.555564
ACGAGCA350.003558748262.1428572
GCACGCA606.768745E-660.4166682
AGCACGC651.0877369E-555.769231
GTAGTAA1152.4519977E-950.4347841
TAGGGTC450.00961833348.3333325
TAGTAAC1307.2559487E-944.6153832
AACACGA657.31032E-444.6153836
AGAAGCG700.001052869541.428575
AGTGATA750.001478106838.6666686
ACACGAG800.002029387736.2500047
ATATAAG850.002732284434.1176451
CTATGTG1102.423272E-432.9545443
GAATACT900.003615432232.222226
GTAACAA1801.276112E-732.222224
CACGAGC950.00471066830.5263188
Produced by FastQC (version 0.11.2)