FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_297.351000000a0ddf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_297.351000000a0ddf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166126
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA3960.23837328292982432No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT3940.2371693774604818No Hit
GTATGTAGAAACCAGCCTGCTGCAACGGCACTAAACACAACAATAACCTC3770.22693618097107013No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGAATTGAAAAGAACCCTT2990.17998386766671082No Hit
CCCATACACTGGTGTTCCCCCTTACTCCCATGGAACGGGAACAGGCCACA2480.14928427819847584No Hit
TCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACACT2220.13363350709702274No Hit
GTATTTAGTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGA2210.13303155436235145No Hit
GCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAATG2190.13182764889300894No Hit
CTTTACATACAGTCCCATCCCCATTCTTTTGGTTTTAGACATCGTTCGAG2050.12340031060761109No Hit
CCCCATATACATCTTCGGAGCTTATGTGTACCCAATCTCACCAAGGTGAG2010.120992499668926No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT2000.12039054693425472No Hit
GGTTTTAGCTTTTCAAGTTCTTCAATGGTTTTGAAATCTCCAAGATCTGG1890.11376906685287072No Hit
TCTCAGGCTTGTCCAATGAATCAATGATATCTTGGCAAAAGGGTACCAAT1880.11316711411819944No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT1840.11075930317951434No Hit
GTTAAAACAGGCCTCAAAAAGGTTGCAACACTTAGCGTAGCATTGTTCCT1810.10895344497550052No Hit
AGCAGAGCCTTTAAGATGAATATAAATCCTTACTTTCTCTTCATAGATGT1790.10774953950615797No Hit
TAGTAGTACAGACCCAAGAGTGGACTTGGGAAAGTGGCCAAAATATACTG1760.10594368130214415No Hit
TTCTAATACCTTACTCCCCTCCTTTTCAAAGCCCAACCATTCATTGAACC1680.10112805942477396No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGCA100.0070855776145.02
TAGTAGA100.0070855776145.03
AGCACGC100.0070855776145.01
GAAACAC850.0119.4117664
AAACACG1000.0101.55
CAGAGCG301.595516E-596.6666643
CGGTGCA406.094888E-790.6252
AACACGA1200.078.5416646
AGCGTTT502.297771E-672.56
CAGCCTT406.6468536E-572.55
GAGCGTT502.297771E-672.55
GGTGCAT502.297771E-672.53
GTAACAA2450.071.020414
AGAAGCG754.822141E-967.6666645
ACACGAG1400.067.321437
GGTATGC451.19094024E-464.444458
GCGTTTT702.3225948E-762.1428577
AAGCGGT606.7807196E-660.4166687
GAGCAGA851.29639375E-859.7058831
AGCAGAA851.29639375E-859.7058832
Produced by FastQC (version 0.11.2)