FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_291.351000000a0dc2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_291.351000000a0dc2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164536
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAA1258.913048E-1152.21
AGAAGCG450.00962493648.3333322
TAGTAAC1351.016997E-842.9629632
GTGTAAC750.001479781838.6666681
CATACTG1604.5498382E-836.255
CTACATA1209.515361E-636.252
TACATAC1251.2586866E-534.83
GCCATTA850.002735361534.117657
AAGAGCG900.00361949332.222229
AGTAACA1902.0587686E-730.5263143
GTTATAG1204.043468E-430.2083341
CTATTAT1504.376678E-529.0000021
GTAACAA2054.0046507E-728.2926834
ACAGCCC1555.472934E-528.0645168
TACTCAG1306.459772E-427.8846175
TTAGGAC1050.007690050627.6190473
TAGTAGA1050.007690050627.6190473
TCTACAT1606.794084E-527.18751
ACTGTCC1358.053887E-426.8518528
ACATACT1358.053887E-426.8518524