FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_286.351000000a0db5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_286.351000000a0db5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114484
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGACT259.3653705E-487.04
AACACGA406.638028E-572.56
GGATAGA350.003558441362.1428576
AGACTTA450.00961750848.3333326
ACACGAG657.309279E-444.6153877
CATAGGC907.4409254E-540.277783
AAACACG907.4409254E-540.277785
TATTAGT800.002029102336.251
CACGAGC850.002731899734.117658
TCTACCC850.002731899734.117653
GAACTAG1102.4227615E-432.954548145
CAGGTTC900.003614925332.222225
TTTACAC900.003614925332.222223
TCTAAAG950.004710009330.5263183
TAACAAG1504.365183E-529.0000025
TTAGAGA1000.00605231129.04
TAGGGCT1000.00605231129.04
GTAGTAA1255.1257224E-429.01
TAGAAAC1804.652389E-628.1944452
GGCATAG1306.4476655E-427.8846171
Produced by FastQC (version 0.11.2)