FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_285.351000000a0da9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_285.351000000a0da9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193737
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC3670.189432065119208No Hit
ATTTCCTTTTATTCATCCATTATTTCATCCACTGATTCCAACACTTTACT3620.18685124679333323No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT3610.18633508312815827No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT3550.18323810113710856No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT3090.15949457253906069No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC2940.15175211756143636No Hit
GCACAGCATTTTCTTGTGAACTTCAAGCACCAGTAAAAGAACTGAAAATC2790.14400966258381206No Hit
AAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAAT2700.1393641895972375No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT2680.13833186226688757No Hit
GTGGAATAGTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTT2540.13110557095443826No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT2510.12955707995891336No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT2490.12852475262856347No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC2470.12749242529821356No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC2470.12749242529821356No Hit
CCTCAGAGCTGATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT2460.1269762616330386No Hit
TTCCTATAATGCACGATAGAACAAAAATTAGACAGCTGCCTAACCTTCTC2420.12491160697233879No Hit
AGCACAGCATTTTCTTGTGAACTTCAAGCACCAGTAAAAGAACTGAAAAT2240.11562066099918963No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA2160.11149135167779No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT2090.10787820602156531No Hit
TTACTACACAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATATGGG2020.10426506036534064No Hit
ACATTGTCTCTGGAGACCATATAAACAACAAAGATAGCTATCATCAGTGT1970.10168424203946588No Hit
TAGTAGTACAGATCCAAGAGTGGACTCAGGAAAGTGGCCAAAATATACTG1950.10065191470911598No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGCA351.893568E-9124.285712
CGAACAG203.861301E-4108.755
CCCTCGA203.861301E-4108.751
GTAACAA2650.0101.226414
GAAACAC1050.096.666674
AAACACG1150.088.260875
CCTCGAA259.375244E-487.02
TAACAAG3150.085.158735
TAGAAAC1400.082.857142
GAGCAGA451.2291221E-680.555561
GTAGAAA1600.072.51
CAAAGTT300.001933378972.5145
CTTTAAG1050.069.047628
TAGTAAC4100.067.195122
ACAAGAG4250.064.8235247
AACACGA1350.064.444446
AGAAGCG350.003562164562.1428575
CGAGCCT350.003562164562.1428575
GAGCATC1200.060.4166684
ACACGAG1500.058.07
Produced by FastQC (version 0.11.2)