FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_284.351000000a0d9c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_284.351000000a0d9c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171996
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT2900.16860857229237888No Hit
TAGTAGTACAGACCCAAGAGTGGACTTGGGAAAGTGGCCAAAATATACTG2360.137212493313798No Hit
CTATAGAACATGCTGGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT2230.12965417800413964No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA2180.126747133654271No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC2170.12616572478429733No Hit
GTATGTAGAAACCAGCCTGCTGCAACGGCACTAAACACAACAATAACCTC2160.1255843159143236No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATTATCGTTTTGT1930.11221191190492802No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA1770.10290936998534848No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAAC259.373439E-487.02
AAGCGGT554.3726686E-879.090917
AGCGGTG554.3726686E-879.090918
GCGGTGC651.3911813E-766.923079
AGAAGCG1009.094947E-1265.255
GAGCAGA1350.064.444441
AGCAGAA1450.060.02
GAAGCGG1102.5465852E-1159.3181846
CAGAAGC1600.054.3750044
TAGCCCT400.006042401354.3750049
ACGTGTC805.844995E-754.3750049
GGTACGT858.8812885E-751.1764686
GTACGTG858.8812885E-751.1764687
TGGTACG858.8812885E-751.1764685
TTGGCCG752.5417046E-548.3333364
TACGTGT901.3171339E-648.3333328
ACTGTCC1255.153197E-946.3999988
CGGCCAT803.7216836E-545.3125049
TACATAC1307.2905095E-944.6153833
ACTGAAC700.001054199641.428578
Produced by FastQC (version 0.11.2)