FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_282.351000000a0d72.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_282.351000000a0d72.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences209620
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT4340.20704131285182711No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT3300.15742772636198835No Hit
TCTGCATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA3170.15122602805075852No Hit
ATACTAAACAGATCAGGACAAGGTATTTGAGCCTTTAGTCTTTTTGTTTT3100.14788665203701937No Hit
GTATGCAGAAACCAACCTGCTGCAACGGCACTGAATACAACAATAACCTC2940.14025379257704418No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT2880.13739147027955348No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT2730.13023566453582675No Hit
GTTTAGTATACCATTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA2680.1278503959545845No Hit
CCCATACACTGGTGTTCCCCCTTATTCCCATGGAACAGGAACAGGCTACA2590.12355691250834844No Hit
GTGTAGCATTTCTATGAACTCAGCTGATGTTGCTCCTGCCACTGGCAAGA2410.11496994561587634No Hit
ATTAGTAGCAGATGGTGGGCCCAACATTTACAATTTGAGAAACTTGCATA2370.11306173075088255No Hit
CTTTTAGGTTGAATGATTTAAATGGAGCCGACAAAGGTGGATTAATACCT2370.11306173075088255No Hit
GAATACAACAATAACCTCTTTTAGGTTGAATGATTTAAATGGAGCCGACA2340.1116305696021372No Hit
ACTAAACACTGATCAGAGGAACATGATTCTTGAGGAACAATGCTACGCTA2190.10447476385841047No Hit
CCTATGGGATGGACTGCAATCTTCTGATGATTTTGCTCTATTTGTTAATG2110.10065833412842287No Hit
GTACTGACCAGCCATTTTCTGAGGAATTATGCTCTCAAATGCTTCAAATG2110.10065833412842287No Hit
GTTGTATTCAGTGCCGTTGCAGCAGGTTGGTTTCTGCATACTGTCCAATC2100.10018128041217442No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAGA301.0986514E-7120.8333361
TAGTAGA301.596497E-596.6666643
GCAAACC259.3763275E-486.99999145
CACGAGC755.4569682E-1177.333338
GAAACAC755.4569682E-1177.333334
AAACACG755.4569682E-1177.333335
AACACGA755.4569682E-1177.333336
GGCGAAA300.001933601172.5145
ACACGAG851.6916601E-1068.23537
GTAACAA702.3255598E-762.1428574
TAGAAAC954.5292836E-1061.0526282
TAGATGT1206.002665E-1154.3754
GTGCCCG1353.6379788E-1253.7037059
AGAAGCG553.2131255E-452.7272762
ACGAGCA553.2131255E-452.7272769
CCATCCA604.9360807E-448.3333329
ACGAGCC604.9360807E-448.3333329
GAAGCGG657.323266E-444.6153873
TCCTGGA907.462187E-540.27778145
TGTGCCC1859.640644E-1139.1891868
Produced by FastQC (version 0.11.2)