FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_280.351000000a0d65.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_280.351000000a0d65.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences247382
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTAGTACAGACCCAAGAGTGGACTTGGGAAAGTGGCCAAAATATACTG4380.17705411064669216No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC3920.15845938669749618No Hit
TCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACACC3730.15077895724021959No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA3240.13097153390303257No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT3040.12288687131642562No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG3030.12248263818709526No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT2980.12046147254044352No Hit
GTATGTAGAAACCAGCCTGCTGCAACAGCACTAAACACAACAATAACCTC2950.11924877315245248No Hit
GTATTTAGTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGA2920.11803607376446143No Hit
CCCATACACCGGTGTTCCCCCTTACTCCCATGGAACGGGAACAGGCCACA2870.1160149081178097No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT2860.11561067498847936No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATTATCGTTTTGT2800.11318527621249727No Hit
ATGTTAATCCATCCCCTGTGGCCCACTTGGCATAATTGGTTTTCTGTAAT2790.11278104308316693No Hit
GTAGTAACAAGAGCCTTTTTTTCATTTTAATCATTTGTTTGTCACATGTA2750.11116411056584553No Hit
CTTTACATACAGTCCCATCCCCATTCTTTTGGTTTTAGACATCGTTCGAG2650.10712177927254206No Hit
ATATTGTCTATGTAGCTCCTCATTCCTTCGAAATTGGAGAAACCAGCTGG2590.10469638049655998No Hit
GTTAAAACAGGCCTCAAAAAGGTTGCAACACTTAGCGTAGCATTGTTCCT2520.10186674859124754No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGCA202.1274045E-6145.02
CGAGAGA100.0070876596145.01
AGCACGC202.1274045E-6145.01
CTATGAT203.8625803E-4108.752
AAACACG1550.098.225815
GAAACAC1600.095.156254
AACACGA1550.093.5483866
ACACGAG1600.090.6257
CACGCAC353.4321536E-582.857143
CACGAGC1850.078.378388
ACGAGCC851.8189894E-1276.76479
ACGCACT406.6532855E-572.54
CGGTGCA502.3011035E-672.52
CAGCCTT651.3937097E-766.923085
CAGGATT350.003563331262.1428575
CAGAAGC1550.056.1290322
TAACAAG3750.056.066675
AGAAGCG651.0912261E-555.7692343
CGCACTT400.006045524554.3755
GTAACAA4200.050.0595254
Produced by FastQC (version 0.11.2)