FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_279.351000000a0d59.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_279.351000000a0d59.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences227097
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA4820.21224410714364345No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT4750.20916172384487686No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT4050.1783378908572108No Hit
GTATGTAGAAACCAGCCTGCTGCAACAGCACTAAACACAACAATAACCTC3670.16160495294962066No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC3630.15984359106461116No Hit
TAGTAGTACAGACCCAAGAGTGGACTTGGGAAAGTGGCCAAAATATACTG3430.15103678163956372No Hit
CTTTACATACAGTCCCATCCCCATTCTTTTGGTTTTAGACATCGTTCGAG3070.1351845246744783No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAGGAAATTTTCGTTAGCTTT2750.1210936295944024No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG2640.11624988441062631No Hit
TCTCAGGCTTGTCCAATGAATCAATGATATCTTGGCAAAAGGGTACCAAT2590.11404818205436444No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGAATTGAAAAGAACCCTT2530.11140613922685019No Hit
GCCCAAGCCATTGTTGCGAAAAATCCGCTTTTACTGGTAGCGTTAGGGCA2390.10524137262931697No Hit
GATTATAGATATACCTACAAATGCCACAGGGGAGATTCCAAAGTGGAAGG2310.10171864885929802No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGTG100.0070872786145.07
GCACAGG259.3773386E-487.02
CGGTGCA608.258212E-1084.5833362
AGCGGAG300.00193380972.51
GAGCAGA809.822543E-1172.51
AACACGA903.6379788E-1272.56
AGAAGCG754.833055E-967.6666645
GGTGCAT754.833055E-967.6666643
ACGAGCC651.3932004E-766.923079
GAAACAC1009.094947E-1265.254
ACACGAG1051.6370905E-1162.142867
AAACACG1051.6370905E-1162.142865
CAGGATT350.00356295562.1428575
AAGCGGT851.2993041E-859.705887
CACGAGC1102.5465852E-1159.318188
GTGTAAA1153.8198777E-1156.739131
GCGGAGC400.006044887554.3752
GAAGCGG953.1250238E-853.4210476
AGCGGTG1004.681715E-850.758
TAACAAG4600.050.434785
Produced by FastQC (version 0.11.2)