FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_274.351000000a0d4c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_274.351000000a0d4c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences220608
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT3480.15774586597040904No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA3000.13598781549173194No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC2670.12102915578764142No Hit
TTCCTATAATGCACGATAGAACAAAAATTAGACAGCTGCCTAACCTTCTC2580.11694952132288947No Hit
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT2570.11649622860458371No Hit
GGCTTGGGCCGTCCCAAAAAACGACAAAAACAAAACAGAAACAAATCCAT2560.11604293588627793No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG2550.11558964316797216No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA2440.11060342326660863No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT2410.10924354511169132No Hit
ATACTAAACAGATCAGGACAAGGTATTTGAGCCTTCAGTCTTTTTGTTTT2400.10879025239338555No Hit
TAGTAGTACAGATCCAAGAGTGGACTCAGGAAAGTGGCCAAAATATACTG2390.10833695967507977No Hit
GTGCTTGTGATTCTAATTCACTCACGTCATTAGCTCCCATTATGACTTCC2330.10561720336524515No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT2330.10561720336524515No Hit
TCATTAAGACGCTCGAAGAGTGAGTTGAGGATCCGATGGCCATCTTCTTC2320.10516391064693936No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC2310.10471061792863359No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC2270.1028974470554105No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCCT100.007087143145.03
ACGTGCC100.007087143145.02
CGGAGCA404.798494E-9108.752
CACGTGC301.5966838E-596.6666641
GTAACAA2000.094.254
AAACACG554.1472958E-1092.2727365
GGAGCAT502.2604581E-887.03
CACAGGA353.431329E-582.857143
CGGTGCA353.431329E-582.857142
TAGAAAC1000.079.752
GAAACAC651.5570549E-978.076934
GAGCATC755.4569682E-1177.333334
AGCATCT950.076.315795
GCACAGG406.65169E-572.52
GAGCAGA851.6916601E-1068.235291
AGAAGCG554.0511277E-665.9090965
GCGGAGC902.8012437E-1064.444441
ACACGAG808.047209E-963.43757
AACACGA808.047209E-963.43756
TAACAAG3150.059.841275
Produced by FastQC (version 0.11.2)