FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_264.351000000a0d3f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_264.351000000a0d3f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135905
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGCTCAGTAAGAACAATT2160.15893454986939407No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTGTCACATGTAT1990.14642581214819175No Hit
GTTCTATAGAGATGGATTTGTATCTAATTTTGCAATGGAAATTCCTTCAT1910.1405393473382142No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTGCAGTAACT1800.13244545822449505No Hit
GTATAGTATGGTTGTCCAATCCATCATCATTTAAAGATGCAGCAGTAATG1670.12287995290828152No Hit
GCAGAGCATCTTCTCAAAAACTGAGGCAAATAGGCCAAAAATGAACAATG1600.11772929619955116No Hit
AGCATATACTGACACGTACCATTCCTATGCAAAAAACATCCTAAGGACAC1580.11625767999705677No Hit
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA1540.11331444759206798No Hit
GTATGTAGAAACCAGCCTGCTGCAACGGCACTAAACACAACAATAACCTC1500.11037121518707922No Hit
TAGTAGTACAGACCCAAGAGTGGACTTGGGAAAGTGGCCAAAATATACTG1470.10816379088333762No Hit
ATATTGTCTATGTAGCTCCTCATTCCTTCGAAATTGGAGAAACCAGCTGG1360.10006990176961848No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGAT100.00708417145.06
TAGATCG100.00708417145.04
CTTAGAT100.00708417145.02
GAAACAC700.093.214294
GTAACAA2050.091.951224
AAACACG750.087.05
CACGTGC300.001932132872.58
TAACAAG2750.071.1818165
GGAGGGC350.003559877262.1428578
TAGTAAC3250.060.230772
AACACGA851.2940291E-859.705886
ACAAGAG3300.059.3181847
AGTAACA3450.056.7391323
CAAGAGG1800.056.388898
GCCTTTA651.0885959E-555.7692347
GCGGAGC651.0885959E-555.7692341
CTAGGTA651.0885959E-555.769234145
CTGTCCC400.006039685654.3759
CGGTGCG400.006039685654.3755
GTAGTAA3800.051.513161
Produced by FastQC (version 0.11.2)