FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_249.351000000a0d22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_249.351000000a0d22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207349
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT3220.15529373182412262No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATCACTGTTTTGT2800.1350380276731501No Hit
GTGTAAATACAATAAGTAATGAGGAAACGAGTAACGAGTTACAGAAAACC2620.1263570116084476No Hit
GTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAGTAAGGACAATT2500.1205696675653126No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT2430.11719371687348384No Hit
TTCCTATAATGCACGATAGAACAAAAATTAGACAGCTGCCTAACCTTCTC2290.11044181548982633No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA2250.10851270080878134No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC2230.10754814346825882No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTGTATCGCATGTAT2200.10610130745747509No Hit
CCTCAGAGCTGATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT2140.10320763543590758No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC2130.10272535676564631No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG2110.10176079942512382No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT2090.10079624208460132No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGCGT100.0070868367145.06
ACGTGCA100.0070868367145.02
CACGTGC151.2286103E-4144.999981
CGGTGCA351.895387E-9124.285712
GAAACAC950.0122.105274
AAACACG1050.0110.476195
ACACGAG1250.092.7999957
AACACGA1250.092.7999956
GTAACAA2500.089.94
GAGCGTT259.376181E-487.07
TAGAAAC1450.085.02
CACGAGC1400.082.857148
GAGCAGA353.430839E-582.857141
ACGAGCC353.430839E-582.857149
TAACAAG3100.072.55
AGCGTTT300.001933571272.499998
GTAGAAA1900.068.684211
AGAAGCG651.3926001E-766.923073
ACGAGCA1400.062.1428579
ACAAGAG3700.060.7432487
Produced by FastQC (version 0.11.2)