FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_137.351000000a0d15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_137.351000000a0d15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences213847
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA4960.23194152828891682No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATTATCGTTTTGT4070.1903229879306233No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTCAGAAATACAATTGGACCGAT3040.14215771088675547No Hit
GTATGTAGAAACCAGCCTGCTGCAACGGCACTAACCTCATTTAGGTTGAA2750.12859661346663737No Hit
GTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCACTGCCTGCTGTA2730.12766136536869818No Hit
GTGTAAATACAATAAGTAATGAGGAAATGAGTAACGAATTACAGAAAACC2700.1262584932217894No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA2620.12251750083003268No Hit
ACATTACGCATATCCCTTTTTATTGTCAACCGGAACTTCCCTTCTTTCTG2610.1220498767810631No Hit
TCATTAAGACGCTCGAAGAGTGAATTGAGGATCCGATGGCCATCTTCTTC2580.12064700463415433No Hit
TCATAGATGTACCCATACAGGCAGCAATTTCAACAACATTCCCATACACC2530.11830888438930638No Hit
TGACTATCCTGGTCAAGACCGCCTAAACAGACTAAAAAGAAAATTAGAGT2440.11410026794858007No Hit
GTATTTAGTATAGATCTGTTCCTTTTAGTTCTCCAGCTATGAGTCCCAGA2300.10755353126300579No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG2260.10568303506712742No Hit
CTCTAAGGGACAATACATTACGCATATCCCTTTTTATTGTCAACCGGAAC2210.10334491482227948No Hit
CACAAGCACTGCCTGCTGTACACTTCAACCACATCCTTCATTTCATACAA2160.10100679457743153No Hit
ATTATGGAGCTGTTAGCTATTATCGTTTTGTGAAACAATATGTTATCATC2150.10053917052846194No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCAA100.0070869913145.09
AGCACGC353.431087E-582.857141
GAAGCGG406.6512206E-572.51
CGCACTT300.001933654272.55
ACGCACT451.19172066E-464.444444
GCACGCA502.006655E-458.02
GCGTTTT400.006044406454.3757
TATTGAC400.006044406454.3754
AACACGA400.006044406454.3756
TAGGAGC450.00962885448.3333327
GTAACAA3000.048.3333324
TAACAAG3100.046.7741935
TAGTAAC3650.041.712332
TTAGCAT700.001054776641.428573
CACGCAC700.001054776641.428573
GCATACC700.001054776641.428576
TAGCATA750.001480776438.6666644
GTGTAAA1901.2732926E-1038.1578941
CAGAAGC1354.947033E-737.5925942
AGTAACA4150.036.6867453
Produced by FastQC (version 0.11.2)