FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_s1_100.351000000a0d08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_s1_100.351000000a0d08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179768
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCCATTTTTCATCTGCAACACAGAAAGTTGGGGGGAGTT2090.11626095856882204No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGAG100.00708606144.99998145
CGCACTT1650.0114.2424245
GTAGTAA3500.0103.5714341
GTAACAA3550.0102.112684
TAGTAAC3550.0100.070432
ACGCACT2050.091.951224
AACACGA1750.087.01
GCACGCA2250.083.777782
ACGAGCG353.429596E-582.857144
AGCACGC2300.081.956521
AGTAACA4950.074.696973
TAACAAG4950.073.232325
TAGTAGT502.2985405E-672.54
AAGAGCG809.822543E-1172.499999
CGAGCAA451.1912057E-464.444453
ACGAGCC451.1912057E-464.444454
CGAGCTT350.003561747762.1428575
ACACGAG2600.058.557692
ACGAGCT753.743753E-758.0000044
CAAGAGC5950.056.050428
Produced by FastQC (version 0.11.2)