FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_pr8_f12.351000000a12d7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_pr8_f12.351000000a12d7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences269406
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG17030.632131429886491No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA10010.37155816871190694No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG8400.31179706465334844No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA5890.21862913223907413No Hit
CCCTCGGTCTGGACATCGAGACAGCCACACGTGCTGGAAAGCAGATAGTG5840.21677319733042325No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA5390.20006978315256527No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC5370.1993274091891049No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT5110.18967654766412032No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG5030.1867070518102789No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA5010.18596467784681855No Hit
CCTATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTA4870.18076806010259608No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT4560.1692612636689606No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT4420.16406464592473813No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC4350.1614663370526269No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT4350.1614663370526269No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT4260.15812565421705532No Hit
GAGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG4210.15626971930840441No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT4200.15589853232667422No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT4150.15404259741802337No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT4130.153300223454563No Hit
CCAAGAACTAGGTGATGCCCCATTCCTTGATCGGCTTCGCCGAGATCAGA3920.14550529683822927No Hit
AGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC3870.1436493619295784No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCTGCTGT3270.1213781430257678No Hit
GACATAATGGATCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCT3240.12026458208057728No Hit
CATATTGAGAGGGTCGGTTGCTCACAAGTCCTGCCTGCCTGCCTGTGTGT3150.11692389924500568No Hit
AGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC3060.11358321640943408No Hit
CAATATGTATAGGCTACCATGCGAACAATTCAACCGACACTGTTGACACA3050.1132120294277039No Hit
ATATTATTGTGTCTCCGGGTTTTAGCAAGGTCCAGTAATAGTTCATCCTC3030.11246965546424356No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA2900.10764422470175125No Hit
TTCTTACACTTTCCATGCATTCATTGTCACACTTGTGGTAGAACTCAAAA2860.10615947677483056No Hit
GTAGAAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTT2830.10504591582964001No Hit
GTTCTTATCTCTTGCTCCACTTCAAGCAATAGATGTAAGGCTTGCATAAA2800.1039323548844495No Hit
ACCCTCGGTCTGGACATCGAGACAGCCACACGTGCTGGAAAGCAGATAGT2790.10356116790271931No Hit
CTCAATATGAGTGCAGACCGTGCTAGAAAAGTGAGATCTTCGAACTCAGC2740.10170523299406845No Hit
CTCTTGCTCCACTTCAAGCAATAGATGTAAGGCTTGCATAAATGTTATTT2710.10059167204887791No Hit
CTGTTAACCTGCTCGAAGACAGCCACAACGGAAAACTATGTAGATTAAAA2700.10022048506714773No Hit
ATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGTTTT2700.10022048506714773No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAAGC100.0070880074145.01
GCGTTTT100.0070880074145.09
GAGCGAA900.0145.01
AAGGGCG100.0070880074145.05
TCCCCCC100.0070880074145.09
AGTAATC100.0070880074145.0145
CGTACCT203.862958E-4108.755
AATACTA404.802132E-9108.752
GCAAAAG7500.0101.499993
AAGGTCG259.3792524E-487.09
AGCAGGC259.3792524E-487.08
GCAGGGT5950.082.857149
AGCAAAA9300.081.854842
AGCGAAA2150.077.558141
AGCAGGG8650.072.0809258
AAGCAGG12600.070.198417
GAGCAAA11350.067.709251
TACTATT651.3941826E-766.923074
CAAAAGC11400.066.776314
GCAGGGG1950.063.2051289
Produced by FastQC (version 0.11.2)