FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_pr8-vww_5-1.351000000a11a2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_pr8-vww_5-1.351000000a11a2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84595
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG4370.5165789940303801No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA2250.2659731662627815No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG2010.23760269519475147No Hit
CCCTCGGTCTGGACATCGAGACAGCCACACGTGCTGGAAAGCAGATAGTG1850.21868904781606477No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA1320.15603759087416516No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG1310.15485548791299722No Hit
CAAGAGGATTGATGACTTTAGTTCTAATGAAACAGAAAAAGTATCTATAA1280.15130917902949348No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT1230.14539866422365388No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA1220.14421656126248597No Hit
CCAAGAACTAGGTGATGCCCCATTCCTTGATCGGCTTCGCCGAGATCAGA1200.14185235534015014No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT1200.14185235534015014No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC1130.13357763461197472No Hit
GAGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG1100.13003132572847095No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA1090.12884922276730304No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT1040.12293870796146344No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT990.11702819315562386No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC980.11584609019445595No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT940.11111767834978427No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT910.10757136946628051No Hit
CCTATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTA900.1063892665051126No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT890.10520716354394469No Hit
GAGTGGAGGTCTCCCATTCTCATTACTGCTTCTCCAAGCGAATCTCTGTA880.10402506058277677No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAGGC100.0070794676145.09
GAGCGAA202.1192245E-6145.01
TCCCCCC100.0070794676145.09
GCCAAAC100.0070794676145.0145
ACCAGAG259.356842E-487.0145
GCAAAAG2400.081.56253
GCAGGGT1950.078.076939
CAAAAGC2550.076.764714
AGCAGGG2400.075.5208368
AAGCAGG3300.068.106067
AGCAAAA3150.064.444442
AGCGAAA451.1875714E-464.444442
GCAGGGG451.1875714E-464.444449
GAGCAAA3300.061.515151
AAGGGTG606.7520323E-660.4166685
CAAGGGT1007.057679E-1058.04
TACTATT501.9996894E-458.04
AAAGCAG4000.056.18756
GGGTATT651.0850579E-555.769237
TAGCACG651.0850579E-555.769237
Produced by FastQC (version 0.11.2)