FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_pr8-vww_100-1.351000000a1188.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_pr8-vww_100-1.351000000a1188.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115481
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG7460.6459937132515305No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA4820.4173846779989782No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG3690.3195330833643629No Hit
CCCTCGGTCTGGACATCGAGACAGCCACACGTGCTGGAAAGCAGATAGTG2880.24939167482096622No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT2470.21388799889159255No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC2210.19137347269247754No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT2200.19050752937712698No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA2180.18877564274642583No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT2140.1853118694850235No Hit
GAGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG2090.18098215290827063No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG2080.18011620959292005No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA2030.17578649301616717No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA2010.174054606385466No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC1920.16626111654731082No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT1680.14547847697889696No Hit
CCAAGAACTAGGTGATGCCCCATTCCTTGATCGGCTTCGCCGAGATCAGA1680.14547847697889696No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT1680.14547847697889696No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA1660.1437465903481958No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT1560.13508715719469006No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT1560.13508715719469006No Hit
AGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC1520.13162338393328774No Hit
CTCTTGCTCCACTTCAAGCAATAGATGTAAGGCTTGCATAAATGTTATTT1510.13075744061793715No Hit
AGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC1450.12556178072583368No Hit
CCTATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTA1450.12556178072583368No Hit
CTGAAAGAAGAATCCGATGAGGCACTTAAAATGACCATGGCCTCTGTACC1390.12036612083373022No Hit
ACCCTCGGTCTGGACATCGAGACAGCCACACGTGCTGGAAAGCAGATAGT1360.1177682908876785No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT1340.11603640425697734No Hit
CATATTGAGAGGGTCGGTTGCTCACAAGTCCTGCCTGCCTGCCTGTGTGT1340.11603640425697734No Hit
GACATAATGGATCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCT1300.11257263099557503No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCTGCTGT1260.10910885773417273No Hit
GTATTCATCACCCGCCTAACAGTAAGGAACAACAGAATCTCTATCAGAAT1230.106511027788121No Hit
TCATTACTCATGTCAAAGGAAGGCACGATCGGGCTCGCTGCCTTTTCGTC1220.1056450844727704No Hit
GTAGAAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTT1190.10304725452671869No Hit
CTCCTATTGTGACTGGGTGTATATTCTGGTAAGGGAGACTGCTGTTTATA1180.10218131121136811No Hit
CAATATGTATAGGCTACCATGCGAACAATTCAACCGACACTGTTGACACA1180.10218131121136811No Hit
TGTCAAAGGAAGGCACGATCGGGCTCGCTGCCTTTTCGTCCGAGAGCTCG1170.10131536789601753No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA1170.10131536789601753No Hit
GTTCTTATCTCTTGCTCCACTTCAAGCAATAGATGTAAGGCTTGCATAAA1160.10044942458066695No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGAC100.0070827976145.0145
CTCCCCC100.0070827976145.08
CGCTCCC100.0070827976145.06
TCGCTCC100.0070827976145.05
AGTTATG151.227211E-4145.02
TCCCCCC100.0070827976145.09
AGCAGGC151.227211E-4145.08
GAGCGAA600.0132.916671
GCAAAAG2950.0110.593223
TTTAAAC203.8573053E-4108.753
TAAACTA203.8573053E-4108.755
CTATTCG203.8573053E-4108.759
TCGTACT451.0830263E-896.6666642
AGCGAAA900.088.6111152
GCAGGGT2400.087.6041649
TTTTTAA259.3655783E-487.01
TTTTAAA259.3655783E-487.02
AAGCAGG5100.079.607857
AGCAAAA4200.077.6785662
AGCAGGG3300.076.8939368
Produced by FastQC (version 0.11.2)