FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_pr8-vww_1-5.351000000a11cb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_pr8-vww_1-5.351000000a11cb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130896
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAGGATTGATGACTTTAGTTCTAATGAAACAGAAAAAGTATCTATAA6210.4744224422442244No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC3000.22918958562522918No Hit
GGTTTTAGCTTTTCAAGTTCCTCAATAGTTTTGAAATCTCCAAGATCCGG2420.18487959907101822No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT2150.16425253636474757No Hit
GCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC1860.1420975430876421No Hit
GTGTAAATACAATAAGTAATGAGGAAACAGGTAACGAATTACAGAAAACC1770.13522185551888521No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT1570.1199425498105366No Hit
ATACTAAACAGATCAGGACAAGGTATTTGAGCCTTCAGTCTTTTTGTTTT1510.11535875809803202No Hit
GCACAGCATTTTCTTGTGAACTTCAAGCACCAACAAAAACTGAAAATCAA1490.11383082752719717No Hit
GTATTTACACAGTCCCTCCACAACTTCCATAAGAAGTTTTCGTTAGCTTT1430.10924703581469258No Hit
GGAATACAACTTAAAACAAATGCTGAAGACATAGGAACCAAAGGTCAAAT1320.10084341767510084No Hit
CCTAAGATGTGGGAGATGGGAACAACCAAAGAACTGGTGCAAAACACCTA1320.10084341767510084No Hit
GGCCAGTACAGTGGATTTGCAAGAGCAGTGCTCAAACAAATGAGAGACCA1310.10007945238968341No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTAAC100.0070838705145.03
GCGGAGC100.0070838705145.01
CGGTGCA450.0128.888892
AGGATTG950.0114.473685
GTAACAA1450.0100.04
GAGCAGA301.5942434E-596.6666641
AAACACG850.093.823535
GGATTGA1250.087.06
TTCGGAT1300.078.07692145
ACGAGCA755.4569682E-1177.333339
CAAAAGC1250.075.44
ACACGAG1000.072.57
CGAGCCT300.001931971272.55
ACGAGCC300.001931971272.54
AACACGA1000.072.56
GAAACAC1150.069.3478244
CACGAGC1050.069.0476158
AGCATCT955.456968E-1268.6842045
ACCGGTC554.0419254E-665.909098
CCGGTCC554.0419254E-665.909099
Produced by FastQC (version 0.11.2)