FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_pr8-vww_1-100.351000000a11e5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_pr8-vww_1-100.351000000a11e5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130864
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAGGATTGATGACTTTAGTTCTAATGAAACAGAAAAAGTATCTATAA5970.4561988018095122No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC3660.2796796674410075No Hit
GGTTTTAGCTTTTCAAGTTCCTCAATAGTTTTGAAATCTCCAAGATCCGG2400.1833965032400049No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT2310.17651913436850472No Hit
GCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC1840.1406039858173371No Hit
GTGTAAATACAATAAGTAATGAGGAAACAGGTAACGAATTACAGAAAACC1760.13449076904267027No Hit
GCACAGCATTTTCTTGTGAACTTCAAGCACCAACAAAAACTGAAAATCAA1760.13449076904267027No Hit
GTATTTACACAGTCCCTCCACAACTTCCATAAGAAGTTTTCGTTAGCTTT1610.12302848759016993No Hit
ATACTAAACAGATCAGGACAAGGTATTTGAGCCTTCAGTCTTTTTGTTTT1590.12150018339650324No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT1540.11767942291233648No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT1510.11538696662183642No Hit
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT1460.11156620613766964No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA1450.11080205404083629No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT1440.11003790194400292No Hit
CCTAAGATGTGGGAGATGGGAACAACCAAAGAACTGGTGCAAAACACCTA1390.10621714145983617No Hit
GTAGAAACACGAGCATTTTTCACTCAATTGTATTTATTGAATTAATGGAT1370.10468883726616947No Hit
GTATAGCACCGGTCCTGTTCTCCAATAAGATAGCAAGATTGGGGAAAGGG1320.10086807678200269No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTATA100.0070838705145.09
GAGCAGA253.693276E-8145.01
GCTTGCA100.0070838705145.09
AACACGT100.0070838705145.01
AGGATTG500.0130.55
GGATTGA700.093.214296
GAGCATC800.090.6254
AGCATCT850.085.294125
AGAAGCG353.4261007E-582.857145
GAAGCGG353.4261007E-582.857146
GTAACAA2100.082.857144
CAGCCTT353.4261007E-582.857145
CGGTGCA554.364483E-879.0909042
ACAACAG554.364483E-879.0909041
TAACAAG2300.075.652175
GCAGAGC1400.072.51
ACAAGAG2500.069.67
CAGAGCA1150.063.0434722
AACTTAA753.7358222E-758.08
GAAACAC651.08837085E-555.769234
Produced by FastQC (version 0.11.2)