FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_pr8-vww_1-10.351000000a11d8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_pr8-vww_1-10.351000000a11d8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96020
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAGGATTGATGACTTTAGTTCTAATGAAACAGAAAAAGTATCTATAA5310.5530097896271611No Hit
GGTTTTAGCTTTTCAAGTTCCTCAATAGTTTTGAAATCTCCAAGATCCGG2760.2874401166423662No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC2280.237450531139346No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT1810.18850239533430535No Hit
GCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC1490.15517600499895856No Hit
CCTAAGATGTGGGAGATGGGAACAACCAAAGAACTGGTGCAAAACACCTA1410.14684440741512186No Hit
GCCTCAAGCATGCTATGTTGACCCACTGGCTTCCTGTATGATGCACTGTT1300.1353884607373464No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT1300.1353884607373464No Hit
GTGTAAATACAATAAGTAATGAGGAAACAGGTAACGAATTACAGAAAACC1300.1353884607373464No Hit
GCACAGCATTTTCTTGTGAACTTCAAGCACCAACAAAAACTGAAAATCAA1180.12289106436159133No Hit
GGCCAGTACAGTGGATTTGCAAGAGCAGTGCTCAAACAAATGAGAGACCA1130.11768381587169341No Hit
GTATTTACACAGTCCCTCCACAACTTCCATAAGAAGTTTTCGTTAGCTTT1120.11664236617371382No Hit
ATACTAAACAGATCAGGACAAGGTATTTGAGCCTTCAGTCTTTTTGTTTT1070.11143511768381587No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT1050.10935221828785668No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA1010.10518641949593835No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGAGAG100.007080952145.07
GAGCGAA100.007080952145.01
AGGATTG750.0135.333345
GGATTGA1050.0103.5714346
GAGCATC301.5920688E-596.6666644
GAAACAC700.093.214294
AGCATCT406.075188E-790.6255
CGGTGCA406.075188E-790.6252
GTAACAA1000.087.04
AACACGA608.1854523E-1084.5833366
GTAAATA702.910383E-1182.857143
TAGAAAC1000.079.752
GTGTAAA755.4569682E-1177.3333361
ACGTGCA406.632575E-572.52
AAACACG1000.072.55
CTTTAAG406.632575E-572.58
ACGAGCA406.632575E-572.59
AACACGT300.001930397872.56
CACGAGC754.793037E-967.666678
TTTAAGA451.1883892E-464.444449
Produced by FastQC (version 0.11.2)