FastQCFastQC Report
Mon 23 Oct 2017
000000000-B56MK_l01n01_pr8-vww_1-1.351000000a11be.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B56MK_l01n01_pr8-vww_1-1.351000000a11be.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78846
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAGGATTGATGACTTTAGTTCTAATGAAACAGAAAAAGTATCTATAA2530.32087867488521926No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG2310.2929761814169394No Hit
GCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAAAAATGAACAATGC1320.16741496080967963No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA1240.15726859954848693No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG1190.1509271237602415No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT1090.1382441721837506No Hit
GGTTTTAGCTTTTCAAGTTCCTCAATAGTTTTGAAATCTCCAAGATCCGG1050.13317099155315423No Hit
CCCTCGGTCTGGACATCGAGACAGCCACACGTGCTGGAAAGCAGATAGTG900.11414656418841793No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA400.0145.01
CGGTGCA203.8527095E-4108.752
GTAACAA406.065002E-790.6254
CGAGCCT259.35446E-487.04
GCAAAAG1100.079.090913
AACGTAT300.001929108572.57
CGTATAG300.001929108572.59
TTCAACG300.001929108572.54
GCAGGGT1000.072.59
ACGAGCC300.001929108572.53
TCAACGT300.001929108572.55
CTATATA554.027015E-665.9090962
TATATAC554.027015E-665.9090963
GGATTGA554.027015E-665.9090966
AGCAGGG1450.065.08
AAGCGGT451.18707125E-464.444455
CAACGTA350.003554325662.142866
TTCGGAT350.003554325662.14286145
AAGCAGG2550.056.8627437
CACGAGC651.0843754E-555.7692342