FastQCFastQC Report
Tue 14 Nov 2017
000000000-B4YN2_l01n02_mcb_f17_adapter6_4g7.352000000a7514.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4YN2_l01n02_mcb_f17_adapter6_4g7.352000000a7514.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4071025
Sequences flagged as poor quality0
Sequence length251
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGGGC28900.0122.923889
GATCGGG28100.094.16371
CGGGAGA32300.089.12544
TCGGGAG22100.084.2533953
GGAGAGG63700.082.6923146
ATCGGGA31550.080.372422
GAGAGGG66700.078.9730157
GGGAGAG65350.077.60525
AAGGGGC29600.076.976359
AGAGGGG75800.074.017158
ACGGGAG17750.062.1126753
GAACGGG27400.059.908761
AACGGGA31150.050.7303352
TCTCGGG33550.044.18033245
AAAGGGG88250.043.308788
GAAAGGG81550.041.0085877
GAGGGGG40550.040.480899
CGGGAAA42500.039.4882354
GGAAAGG90200.038.0266086
TAGGGAG23950.037.849693
Produced by FastQC (version 0.11.2)