FastQCFastQC Report
Tue 14 Nov 2017
000000000-B4YN2_l01n02_mcb_f17_adapter4_3g6.352000000a7507.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4YN2_l01n02_mcb_f17_adapter4_3g6.352000000a7507.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3299121
Sequences flagged as poor quality0
Sequence length251
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGG25250.0130.99011
GAGGGGC22000.0115.8181849
TCGGGAG22300.0102.724223
ATCGGGA32500.099.50772
CGGGAGA28850.086.620454
GAGAGGG50200.082.72417
GGGAGAG56050.079.991085
GGAGAGG53500.078.995336
AAGGGGC22650.078.421639
GATTGGG33900.065.766961
AGAGGGG66300.063.005288
GAACGGG18150.059.3939361
TCGGGAA30900.057.8802573
CGGGAAA35700.057.303924
GAAAGGG65550.050.457667
GATAGGG23000.049.532611
GGTCGGG15400.046.136361
GGAAAGG71750.045.926836
AAAGGGG74400.045.1142468
CGGGAGG11600.044.3534474
Produced by FastQC (version 0.11.2)