FastQCFastQC Report
Tue 14 Nov 2017
000000000-B4YN2_l01n02_mcb_f17_adapter2_2g8.352000000a74f9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4YN2_l01n02_mcb_f17_adapter2_2g8.352000000a74f9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3750415
Sequences flagged as poor quality0
Sequence length251
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATATATATATATATATATATATATATATATATAT2362886.300316098351782No Hit
TATATATATATATATATATATATATATATATATATATATATATATATATA1766594.710385384017502No Hit
ATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG129950.34649498788800703Illumina Single End PCR Primer 1 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA99910.2663971853781515Illumina Single End PCR Primer 1 (100% over 34bp)
ATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAG69230.18459290505184095Illumina Single End PCR Primer 1 (100% over 30bp)
ATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGA62010.1653417021849582Illumina Single End PCR Primer 1 (100% over 36bp)
ATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAG58780.15672932195503697Illumina Single End PCR Primer 1 (100% over 28bp)
ATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAA55080.14686374707865663Illumina Single End PCR Primer 1 (100% over 26bp)
TATATATATATATATATATATATATATATATATATATATATATATATAAG49820.13283863252466727No Hit
ATATATATATATATATATATATATATAGATCGGAAGAGCGTCGTGTAGGG45220.1205733232188971Illumina Single End PCR Primer 1 (100% over 24bp)
ATATATATATATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC39820.10617491664255821Illumina Single End PCR Primer 1 (100% over 38bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGGGC27650.0128.923349
GGAGAGG60750.087.7155766
AAGGGGC26000.087.634139
GAGAGGG73300.072.5303047
GATCGGG27800.071.825151
TCGGGAG26550.071.5156253
AGAGGGG82900.069.1552358
CGGGAGA39600.065.580454
ATCGGGA31950.061.345522
GGTCGGG15200.059.6378361
GAACGGG18050.055.6506651
CGGAGAG27150.055.045745
GTCGGGA16900.050.7393652
ACGGGAG17250.049.7098733
GAAAGGG66750.048.6326947
CGGGAAA35600.048.1738974
GATTGGG34750.046.5321241
GGTCCGG8250.046.0300561
AAAGGGG78150.045.143718
GATCCGG16200.044.6139561
Produced by FastQC (version 0.11.2)