FastQCFastQC Report
Tue 14 Nov 2017
000000000-B4YN2_l01n02_mcb_f17_adapter1_ig4.352000000a74ec.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4YN2_l01n02_mcb_f17_adapter1_ig4.352000000a74ec.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4307155
Sequences flagged as poor quality0
Sequence length251
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGG39550.051.106194245
GAGGGGC17600.039.6732989
CGGTGGG47200.029.327332245
GCGTCGG27150.027.974218245
AGAGGGG42600.025.3051648
GAGAGGG40400.024.2574277
AAGGGGC22350.024.1163339
TCGCCGG47250.022.296297245
GAACGGG20350.021.6707631
GGAGAGG45350.021.0694626
GGGAGAG43400.020.8870985
CGGGAGA24100.019.8236524
GGGGGGC31200.019.23878340-44
GGGGGCC18850.016.89655340-44
GAGGGGG25050.016.6267479
GATCGGG18500.016.5540541
GTGGTCC20400.016.213236245
ACGGGAG19150.015.99216653
AAAGGGG51750.014.9130448
GGGGGCG26800.014.9011240-44
Produced by FastQC (version 0.11.2)