FastQCFastQC Report
Tue 14 Nov 2017
000000000-B4YN2_l01n01_mcb_f17_adapter6_4g7.351000000a7517.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4YN2_l01n01_mcb_f17_adapter6_4g7.351000000a7517.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4071025
Sequences flagged as poor quality0
Sequence length251
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC884142.171787203468414TruSeq Adapter, Index 12 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCGG24150.030.4347611
AATCGGA40250.018.8695532
GAGCACA2840100.09.2604959
AGAGCAC2853000.09.2315048
CGGAAGA2889300.09.1960794
TCGGAAG2890900.09.19098953
GAAGAGC2884450.09.1818136
GATCGGA2919650.09.1718121
GGAAGAG2910350.09.1421935
ATCGGAA2938200.09.1139072
AAGAGCA2919050.09.0897667
GGATCGG17708.462961E-68.9971691
GTCGGAA26750.0089875535.4953231
CCAGTCC37454.6286866E-45.2336416245
AGCGATG33200.0031270925.1656593
CATCCCC40402.4296762E-45.154699245
Produced by FastQC (version 0.11.2)