FastQCFastQC Report
Tue 14 Nov 2017
000000000-B4YN2_l01n01_mcb_f17_adapter4_3g6.351000000a750a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4YN2_l01n01_mcb_f17_adapter4_3g6.351000000a750a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3299121
Sequences flagged as poor quality0
Sequence length251
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC1021703.0968855037447853TruSeq Adapter, Index 6 (100% over 50bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG43250.13109552514139372TruSeq Adapter, Index 6 (97% over 49bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCGG23300.059.409871
AATCGGA35000.039.552
GGATCGG10850.027.0967731
TCGGAAG2500100.011.5733373
CGGAAGA2494500.011.5354794
AGAGCAC2467700.011.4919768
GATCGGA2521150.011.4912831
GAAGAGC2493050.011.478316
GAGCACA2463900.011.4748979
ATCGGAA2538700.011.4745732
GGAAGAG2512400.011.4191615
AAGAGCA2521800.011.3134477
AGCGGTG16102.7110465E-48.3695654
CGGAAGG25301.4893612E-67.74703554
GGAGCAC22351.8806737E-57.67337859
GATCGGG21208.119818E-57.51179271
GATTCGG18059.240131E-47.4653741
ATTCGGA26352.7435399E-67.43832972
CGGAAAG43904.3655746E-117.2551254
GACGGAA23854.446427E-57.19077541
Produced by FastQC (version 0.11.2)