FastQCFastQC Report
Tue 14 Nov 2017
000000000-B4YN2_l01n01_mcb_f17_adapter2_2g8.351000000a74fc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4YN2_l01n01_mcb_f17_adapter2_2g8.351000000a74fc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3750415
Sequences flagged as poor quality0
Sequence length251
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATATATATATATATATATATATATATATATATATATATATATATAT2466156.575672292266323No Hit
TATATATATATATATATATATATATATATATATATATATATATATATATA1811434.829945486032879No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC855312.280574283112669TruSeq Adapter, Index 4 (100% over 50bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC125490.3346029706045864Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGT98520.2626909288705383TruSeq Adapter, Index 4 (100% over 50bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC96300.2567715839447101Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG66320.1768337637301472Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG59310.15814249889678875TruSeq Adapter, Index 2 (97% over 35bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC57950.15451623353682192Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT55760.14867687975864005Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TATATATATATATATATATATATATATATATATATATATATATATATAAG49960.1332119245470168No Hit
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA46910.12507949120297354Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATATCGTATGC38770.10337522647493678TruSeq Adapter, Index 4 (98% over 50bp)
ATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC37970.10124212920436806TruSeq Adapter, Index 4 (100% over 37bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCGG20000.032.4622651
AATCGGA38500.017.1816962
TCGGAAG2516250.010.0774243
GATCGGA2526050.010.0722731
CGGAAGA2525050.010.0083444
ATCGGAA2548500.09.9931592
GAAGAGC2526500.09.94926556
GGAAGAG2544500.09.8885135
AAGAGCA2588250.09.7260967
GAGCACA2581700.09.6890899
AGAGCAC2586700.09.67983158
CAAGGCG15400.00161408257.95448881
GTCGGAA19803.0307338E-47.4241891
GACGGAA23300.00193369066.3089671
GGGAAGC22300.0085114616.04255722
CCCTACC38156.0105027E-45.1376467245
Produced by FastQC (version 0.11.2)