FastQCFastQC Report
Tue 14 Nov 2017
000000000-B4YN2_l01n01_mcb_f17_adapter1_ig4.351000000a74ef.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4YN2_l01n01_mcb_f17_adapter1_ig4.351000000a74ef.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4307155
Sequences flagged as poor quality0
Sequence length251
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC243440.5651990699197034TruSeq Adapter, Index 2 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGG5100.00489999214.4117595
GCAGGGG10700.00610577559.1588755
TACCCCC30554.4020817E-77.217674245
CACACGC31657.9205347E-76.9668226245
CCTACAC46802.4450492E-65.4967933245
GTTATGT43602.447854E-55.33830071
TAACCTG33150.00306890585.17345243
TGTCAGC31050.0080757125.1288233
Produced by FastQC (version 0.11.2)