FastQCFastQC Report
Thu 26 Oct 2017
000000000-B4Y9M_l01n02_negative-2.352000000a2d50.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y9M_l01n02_negative-2.352000000a2d50.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5367
Sequences flagged as poor quality0
Sequence length151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATC1312.440842183715297Illumina Single End PCR Primer 1 (97% over 35bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGAGA253.2257958E-8145.04
CGGAGAG359.094947E-12145.05
GGATTGG253.2257958E-8145.01
AGGAAAG151.1632714E-4145.05
GATTGGA253.2257958E-8145.02
CGGAAAG151.1632714E-4145.05
ATCCGGA151.1632714E-4145.02
TTGGAGA151.1632714E-4145.04
GAAGGGC151.1632714E-4145.09
GTAGACG100.006896311144.999987
GGATGGG100.006896311144.999981
ACCGGAG100.006896311144.999983
CCGGAAG100.006896311144.999984
GGACCGG100.006896311144.999981
GTCCGGA100.006896311144.999982
ATTGGAG100.006896311144.999983
ATTGGAA201.940949E-6144.999983
GGATCGG100.006896311144.999981
CGAAGAA100.006896311144.999985
AAGGGGC201.940949E-6144.999989
Produced by FastQC (version 0.11.2)