FastQCFastQC Report
Thu 26 Oct 2017
000000000-B4Y9M_l01n02_negative-1.352000000a2c2a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y9M_l01n02_negative-1.352000000a2c2a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7324
Sequences flagged as poor quality0
Sequence length151
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATC1411.9251774986346257Illumina Single End PCR Primer 1 (97% over 35bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATT200.2730748225013654Illumina Single End PCR Primer 1 (97% over 35bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATA150.20480611687602404Illumina Single End PCR Primer 1 (97% over 35bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGAGC100.1365374112506827Illumina Single End PCR Primer 1 (97% over 35bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGAAC80.10922992900054614Illumina Single End PCR Primer 1 (97% over 35bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGGAGATC80.10922992900054614Illumina Single End PCR Primer 1 (97% over 35bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGGA100.006948329145.02
CCGGAAA100.006948329145.04
CGGAGAG253.351306E-8145.05
CGGAGAA201.9906856E-6145.05
GGTCCGG151.1809738E-4145.01
GTCCGGC100.006948329145.02
TACGGAG151.1809738E-4145.03
TCGGAGA253.351306E-8145.04
TGGAGAG100.006948329145.05
CGGAAAG151.1809738E-4145.05
TCCGGCA100.006948329145.03
ATCGGGA100.006948329145.02
ATTCGGA151.1809738E-4145.02
TTCCAAG100.006948329145.03
GATTCGG151.1809738E-4145.01
GATTCGC100.006948329145.01
AAGCGGC100.006948329145.09
GATCAGG100.006948329145.01
TCCAAGA100.006948329145.04
AAACGGA100.006948329145.02
Produced by FastQC (version 0.11.2)