FastQCFastQC Report
Thu 26 Oct 2017
000000000-B4Y9M_l01n02_mr755100000-1.352000000a2c1d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y9M_l01n02_mr755100000-1.352000000a2c1d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19343
Sequences flagged as poor quality0
Sequence length151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATC1140.5893604921677092Illumina Single End PCR Primer 1 (96% over 33bp)
GTCAGACTGCGACAGTTCGAGTCTGAAGCGAGAGCTAACAACAGTATCAA370.1912836685105723No Hit
GCCCACCTGATTGAGATGAAAACATGTGAATGGCCAAAGTCTCACACATT240.12407589308793879No Hit
ATCCATTCTCCCTTTCCATGGATTGACCAGGTGGTTCTCCCAGTTGGTAC230.11890606420927466No Hit
GGGAGATCTTTGCTTCTCTGGCTCGGGTATGAGCACCACCAGTAATAAAA200.10339657757328233No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGGC100.0070374557145.01
GTCTGGA151.21154335E-4145.02
CGAAAAG100.0070374557145.05
GGACTGG151.21154335E-4145.01
GACCGGA100.0070374557145.02
ATTACCT100.0070374557145.0145
CCGGAAG100.0070374557145.04
ATTCGGA151.21154335E-4145.02
AAGCGGG100.0070374557145.09
AAGCGGC253.5735866E-8145.09
GTGGACG100.0070374557145.07
GTTCGGA100.0070374557145.02
TCCGTAA151.21154335E-4145.03
TTCGGAG253.5735866E-8145.03
GTCCCAA100.0070374557145.02
TGGGCAA100.0070374557145.04
GGACCGG100.0070374557145.01
TTGCAGA100.0070374557145.04
ATACGGA100.0070374557145.02
GGGATAG100.0070374557145.05
Produced by FastQC (version 0.11.2)