FastQCFastQC Report
Thu 26 Oct 2017
000000000-B4Y9M_l01n02_mr75510-2.352000000a2d36.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-B4Y9M_l01n02_mr75510-2.352000000a2d36.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7397580
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAGACTGCGACAGTTCGAGTCTGAAGCGAGAGCTAACAACAGTATCAA360440.4872404218676919No Hit
GCGGAAGTCACTGGAAACAGTCCTCGGCTTGACGTGGCACTGGATGAGAG175510.23725326390522306No Hit
GGGAGATCTTTGCTTCTCTGGCTCGGGTATGAGCACCACCAGTAATAAAA169670.22935879030710044No Hit
CTTGTCTTGAAATGATGTCCATAACTGTTTTTCCTCCTTCAGCTGGTCTG160340.2167465576580449No Hit
TGCGATTTCATTTCCGTTTCTCACGCTTGGAACGAACCAAACTGTTTTCC149060.2014983278315341No Hit
CAGACTGCGACAGTTCGAGTCTGAAGCGAGAGCTAACAACAGTATCAACA147020.1987406692458885No Hit
GCTGAGTGGACCAGCTAAAGACTTGGGTATGATAAGATCACTTTCTTCTA138300.186953030585678No Hit
GTGTGAGTCAGACTGCGACAGTTCGAGTCTGAAGCGAGAGCTAACAACAG117720.15913312191284176No Hit
CCTCAATCCACACTCTATTCCACACCATGAGCATGTCCTCAGTGGTCATC108880.14718326804171095No Hit
GCCGGGAAGGCCATTTCGTTTGCTACCACATTGGGAGTGAACAAGTGCCA108490.14665606860621988No Hit
CTCTGGTGTTGTGGTGGCTGAGTGGACCAGCTAAAGACTTGGGTATGATA106280.14366860513843718No Hit
GCCGCGCACAGTGATCTGGGCTATTGGATTGAAAGTGAAAAGAATGACAC105980.14326306711113634No Hit
GGCTGAGTGGACCAGCTAAAGACTTGGGTATGATAAGATCACTTTCTTCT105200.1422086682401542No Hit
GTAAATCTCAAAAAGGCTAGTATCGCCAAAACCATTCTGATGGGTCCATG103880.1404243009200306No Hit
CAGGAACCCTAACAAACCTGGAGATGAGTACATGTATGGAGGTGGGTGTG101980.1378558934137921No Hit
CTGGTGTTGTGGTGGCTGAGTGGACCAGCTAAAGACTTGGGTATGATAAG98100.13261093492736814No Hit
GGCAGCTCCTTTATTTCCACAGAAGAGACCTTCGACTGATGGCTAATGCC97370.13162412572760282No Hit
CGCGCACAGTGATCTGGGCTATTGGATTGAAAGTGAAAAGAATGACACAT92210.12464887165802871No Hit
GGCCCTTTCACTTGGGTTCTGTAACCCTCTCTGGTGTTGTGGTGGCTGAG91530.12372965212948017No Hit
TGTGAGTCAGACTGCGACAGTTCGAGTCTGAAGCGAGAGCTAACAACAGT90470.12229675109968396No Hit
GTGCTTACAACACTCCGGGTGTGGACCCTTCCTCACCCAGGTAGCGGACT90080.12176955166419288No Hit
CGCGGAAGTCACTGGAAACAGTCCTCGGCTTGACGTGGCACTGGATGAGA89760.12133697776840534No Hit
CCGCTTTCCAGCCTTTGTCAGACAGGCTGCGATTTCATTTCCGTTTCTCA89020.12033665063439665No Hit
GGGAAAGAGTGTGGACATGTACATTGAAAGAGCAGGTGACATCACATGGG86530.11697068500779984No Hit
GGAGATCTTTGCTTCTCTGGCTCGGGTATGAGCACCACCAGTAATAAAAA86350.11672736219141934No Hit
CCTGGACATTCCTCAAACCGGATTTCAAGCTCTTCACTGTGCCATGGCCC84830.11467263618642853No Hit
CCGGTGTCTGCCCCAGCATGCCAAGGCAATGGGATGTCATGAAACCACTC84700.11449690304126485No Hit
GCCACGTCAAGCCGAGGACTGTTTCCAGTGACTTCCGCGTCCTTTTCCCA84520.11425358022488435No Hit
GGGAGAACCACCTGGTCAATCCATGGAAAGGGAGAATGGATGACCACTGA84350.11402377534274723No Hit
GCGATTTCATTTCCGTTTCTCACGCTTGGAACGAACCAAACTGTTTTCCC83670.1131045558141987No Hit
GTTTGCTACCACATTGGGAGTGAACAAGTGCCACGTACAGATCATGGACC83430.11278012539235804No Hit
CGGGAAGATGGGCTTTGGAATGGTAACCCTTGGGGCCAGTGCATGGCTCA82720.11182035206107944No Hit
GTTCATGCCACAGATGGCCATCAGGACCACCTTGAGTATGATCTCTCTCA81240.10981969779306204No Hit
GTGAGTCAGACTGCGACAGTTCGAGTCTGAAGCGAGAGCTAACAACAGTA80630.10899510380421706No Hit
CCCGCGTCTAGTGATCTCTGCTGCCATGGCTGTAGTCAGCAGGAGGCCAA80530.10885992446178344No Hit
GTCGTTCTCCTCAATCCACACTCTATTCCACACCATGAGCATGTCCTCAG78790.1065078039034387No Hit
CACAGATGGCCATCAGGACCACCTTGAGTATGATCTCTCTCATGGGTGGA77940.10535877949275305No Hit
CTTTGCTTCTCTGGCTCGGGTATGAGCACCACCAGTAATAAAAACACAAC76970.10404753987114704No Hit
TTCGTCTTGATGCGGAATAAGGGCATCGGGAAGATGGGCTTTGGAATGGT75960.10268222851256763No Hit
GCGGAATAAGGGCATCGGGAAGATGGGCTTTGGAATGGTAACCCTTGGGG75770.10242538776194378No Hit
CAGAACTCTGGCGTTGGCCGTGATTAAATACACATACCAAAACAAAGTGG74840.10116821987731121No Hit
GCCGAGGTAAGATCCTATTGCTACGAGGCATCGATATCGGACATGGCTTC74640.10089786119244401No Hit
CCGTACACAACCCAAGTTGATGTCGTGTTGCACCAGCAATCGACATCATC74450.10064102044182016No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCGGT1950.0100.384631
CCCGTAC1650.083.4848561
CGCTAAT1400.077.678581
CGGTGCT4800.077.031251
GCCGCGT4100.072.51
TCTTATT7450.071.0402769
TCTCTTA7800.068.782067
GTCCGGT1200.066.4583361
GCGGGCT2600.064.134621
CGGCCCA2500.063.81
GCCGGCT2400.060.4166681
CGCCTAT606.8098852E-660.4166681
ACCGTAC606.8098852E-660.4166681
CGGTGTT1950.059.4871861
CGGGGAG10150.059.2857171
GTCCGTA1154.0017767E-1156.7391321
CGTCCAT3500.055.9285741
CTCCGTA1700.055.4411771
CCCCGGT2650.054.7169841
CGCGGAT805.8788646E-754.3750041
Produced by FastQC (version 0.11.2)